Journal: Hepatitis Monthly

Article Title: Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

doi: 10.5812/hepatmon.6767

Figure Lengend Snippet: Nested PCR Amplification of 5’ UTR of the HCV Isolates. The 234 Band is the Biotinylated Inner Product. A: 100 bp DNA Ladder, B-C: Positive Control, D: Negative Control, E-F: Samples With Positive Results.

Article Snippet: The cycling protocol included denaturation at 94˚C for 1 min, followed by annealing at 55.5˚C for 1 min, and extension at 72°C for 1 min. A final extension was performed at 72°C for 5 min. A second PCR was performed with a set of inner biotinylated primers ( ) (M3 and M4) (10 pmol/μl) (Metabion GmbH, Germany) using 1 μl of the first PCR product as DNA template.

Techniques: Nested PCR, Amplification, Positive Control, Negative Control

Journal: Hepatitis Monthly

Article Title: Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

doi: 10.5812/hepatmon.6767

Figure Lengend Snippet: A strip Demonstrating the Presence of Genotype 3a Based on Reaction Among P12, P13, and P14 Specific Probes, and Corresponding Biotinylated Inner Product.

Article Snippet: The cycling protocol included denaturation at 94˚C for 1 min, followed by annealing at 55.5˚C for 1 min, and extension at 72°C for 1 min. A final extension was performed at 72°C for 5 min. A second PCR was performed with a set of inner biotinylated primers ( ) (M3 and M4) (10 pmol/μl) (Metabion GmbH, Germany) using 1 μl of the first PCR product as DNA template.

Techniques: Stripping Membranes

Journal: Hepatitis Monthly

Article Title: Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

doi: 10.5812/hepatmon.6767

Figure Lengend Snippet: A Strip Demonstrating the Presence of Genotype 1a Based on Reaction Between P3 and P4 Specific Probes and Corresponding Biotinylated Inner Product.

Article Snippet: The cycling protocol included denaturation at 94˚C for 1 min, followed by annealing at 55.5˚C for 1 min, and extension at 72°C for 1 min. A final extension was performed at 72°C for 5 min. A second PCR was performed with a set of inner biotinylated primers ( ) (M3 and M4) (10 pmol/μl) (Metabion GmbH, Germany) using 1 μl of the first PCR product as DNA template.

Techniques: Stripping Membranes

Journal: Hepatitis Monthly

Article Title: Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping

doi: 10.5812/hepatmon.6767

Figure Lengend Snippet: A Strip Demonstrating the Presence of Genotype 1b Based on Reaction Between P5 Specific Probe and Corresponding Biotinylated Inner Product.

Article Snippet: The cycling protocol included denaturation at 94˚C for 1 min, followed by annealing at 55.5˚C for 1 min, and extension at 72°C for 1 min. A final extension was performed at 72°C for 5 min. A second PCR was performed with a set of inner biotinylated primers ( ) (M3 and M4) (10 pmol/μl) (Metabion GmbH, Germany) using 1 μl of the first PCR product as DNA template.

Techniques: Stripping Membranes

Journal:

Article Title: Dysregulated expression of IFN-? and IL-10 and impaired IFN-?-mediated responses at different disease stages in patients with genital herpes simplex virus-2 infection

doi: 10.1046/j.1365-2249.2003.02183.x

Figure Lengend Snippet: Analysis of HSV copy number in the genital swabs obtained from HSV-infected individuals in AD and NAD categories. HSV was quantified by HSV-type specific PCR in genital swabs (n = 34) from AD and NAD individuals. The HSV was detected in the DNA isolated from clinical samples by PCR analysis using the Digene Sharp Signal System with HSV-1 and HSV-2 primers, as described in Subjects and Methods section.

Article Snippet: For quantification of HSV copies, the 5′ end HSV primers were biotinylated (Digene Diagnostics Inc.).

Techniques: Infection, Isolation